产品货号：
GS4387
中文名称：
重组人LR3 IGF-1
英文名称：
Recombinant Human LR3 Insulin-like Growth Factor-1
产品规格：
1mg
发货周期：
1～3天
产品价格：
询价
IGF-1 belonged to the insulin gene family,is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle,bone,and cartilage tissue in vitro.It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion.The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition,growth hormone insensitivity,lack of growth hormone receptors,or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B.The LR3 IGF-1 is a long-term analog of human IGF-1,specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.
Storage
This lyophilized preparation is stable at 2-8℃,but should be kept at -20℃ for long term storage,preferably desiccated Upon reconstitution,the preparation is stable for up to one
week at 2-8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 9.1kDa,a single non-glycosylated polypeptide chain containing 83 amino acids.
Purity
>98% by SDS-PAGE analyses.
>90% by RP-HPLC analyses.
Source
Escherichia coli.
Biological Activity
Assay 1:Fully biologically active when compared to standard.
Measured in a serum-free cell proliferation assay using human MCF-7 cells.The ED50 for this effect is 0.3-1.5ng/ml,corresponding to a specific activity of> 6.7×105IU/mg.
Assay 2:Fully biologically active when compared to standard.The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10ng/ml,corresponding to a specific activity of> 1.0×105 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB,pH7.2.
Endotoxin
Less than 0.01 EU/μg of rHuLR3 IGF-1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
MFPAMPLSSL FVNGPRTLCG AELVDALQFV
CGDRGFYFNK PTGYGSSSRR APQTGIVDEC
CFRSCDLRRL EMYCAPLKPA KSA
相关搜索：重组人LR3 IGF-1，LR3 IGF-1, Media Grade, Human
Storage
This lyophilized preparation is stable at 2-8℃,but should be kept at -20℃ for long term storage,preferably desiccated Upon reconstitution,the preparation is stable for up to one
week at 2-8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 9.1kDa,a single non-glycosylated polypeptide chain containing 83 amino acids.
Purity
>98% by SDS-PAGE analyses.
>90% by RP-HPLC analyses.
Source
Escherichia coli.
Biological Activity
Assay 1:Fully biologically active when compared to standard.
Measured in a serum-free cell proliferation assay using human MCF-7 cells.The ED50 for this effect is 0.3-1.5ng/ml,corresponding to a specific activity of> 6.7×105IU/mg.
Assay 2:Fully biologically active when compared to standard.The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10ng/ml,corresponding to a specific activity of> 1.0×105 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB,pH7.2.
Endotoxin
Less than 0.01 EU/μg of rHuLR3 IGF-1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
MFPAMPLSSL FVNGPRTLCG AELVDALQFV
CGDRGFYFNK PTGYGSSSRR APQTGIVDEC
CFRSCDLRRL EMYCAPLKPA KSA
相关搜索：重组人LR3 IGF-1，LR3 IGF-1, Media Grade, Human